scAmpi-A versatile pipeline for single-cell RNA-seq analysis from basics to clinics.

scAmpi-A versatile pipeline for single-cell RNA-seq analysis from basics to clinics.

Blog Article

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful technique to decipher tissue GREENS BERRY composition at the single-cell level and to inform on disease mechanisms, tumor heterogeneity, and the state of the immune microenvironment.Although multiple methods for the computational analysis of scRNA-seq data exist, their application in a clinical setting demands standardized and reproducible workflows, targeted to extract, condense, and display the clinically relevant information.To this end, we designed scAmpi (Single Cell Analysis mRNA pipeline), a workflow that facilitates scRNA-seq analysis from raw read processing to informing on sample composition, clinically relevant gene and pathway alterations, and in silico identification of personalized candidate drug SUPER B COMPLEX treatments.

We demonstrate the value of this workflow for clinical decision making in a molecular tumor board as part of a clinical study.